Advances In Biochemical Engineering - Biotechnology - Modern

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The porosity of the monolith is about 70% and hence inferior to that of the Chromolith column, which typically has 80% porosity. 2 Soft Organic Gel-Based Monoliths Continuous beds made of swollen polyacrylamide gel compressed in the shape of columns were first introduced by Hjerten and his group [33, 42]. Their technology relies on the polymerization of suitable monomers and ionomers directly in the chromatographic column. In the presence of salt, the polymer chains formed aggregate into large bundles by hydrophobic interaction, creating voids between the bundles (irregularly shaped channels) large enough to permit a high hydrodynamic flow at low backpressure.

Therefore, the most important operational variables are the analyte-sorbent interactions that can be modulated by the chemistry of the packing, composition of the mobile phase, and temperature. In contrast, the CEC column has a dual role as it serves as both (i) a flow driving device and (ii) separation unit at the same time. Although the set of variables typical of HPLC is also effective in CEC, their changes may affect in one way or another both column functions. Therefore, optimization of the separation process in CEC is more complex than in HPLC.

Copyright 1998 American Chemical Society). d. 2 MPa, injection, 5 kV for 3 s Capillary Electrochromatography: a Rapidly Emerging Separation Method 39 erably exceed those of the original buffer solution (5 mmol/l). As a result of this increase in ionic strength, the conductivity of the mobile phase increases, and much higher currents are observed. Since the electroosmotic flow is reciprocally proportional to the concentration of ions in the mobile phase the flow velocity decreases dramatically at high pH values [149].

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