Biosynthesis by E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul

By E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul D. Shaw (eds.)

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J. BioI. Chern. 220,477 (1956). STRATTON, CH. , and M. C. REBSTOCK: A new metabolite of Streptomyces venezuelae D-threo-1-p-aminophenyl-2-dichloroacetamido-1 ,3-propanediol. Arch. Biochem. Biophys. 103, 159 (1963). , T. TAZAKI, H. OKAMI, and S. FUKUYAMA: Isolation of a crystalline antibiotic substance from a strain of streptomyces and its identity with chloromycetin. Japan. Med. J. 1, 358 (1948). VINING, L. , and D. W. S. WESTLAKE: Biosynthesis of the phenylpropanoid moiety of chloramphenicol. Can.

McLEAN, M. A. PENNER, and J. EHRLICH: Chloromycetin: biological studies. J. Bacteriol. 55, 425 (1948). SRINIVASAN, P. R, H. SHIGEURA, M. SPRECHER, D. B. SPRINSON, and B. D. DAVIS: The biosynthesis of shikimic from D-glucose. J. BioI. Chern. 220,477 (1956). STRATTON, CH. , and M. C. REBSTOCK: A new metabolite of Streptomyces venezuelae D-threo-1-p-aminophenyl-2-dichloroacetamido-1 ,3-propanediol. Arch. Biochem. Biophys. 103, 159 (1963). , T. TAZAKI, H. OKAMI, and S. FUKUYAMA: Isolation of a crystalline antibiotic substance from a strain of streptomyces and its identity with chloromycetin.

The limiting action of exogenous chloramphenicol could not be ascribed to a general toxic action of the antibiotic on the streptomycete. The antibiotic is rapidly secreted from the hyphae and S. venezuelae never accumulates chloramphenicol in appreciable concentrations. Between 12 and 72 hr. 4 (Lg/g dry weight. At 72 hr. the period of maximum concentration in the fermentation culture, the amount in the medium was 13,000 X that in the total quantity of mycelium in the culture vessel. Precursors An examination of the structure of chloramphenicol (Fig.

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