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Additional info for Comprehensive Natural Products II Vol.6 Carbohydrates, Nucleosides & Nucleic Acids
Substrates 32 and 33 were incubated with GalT and SiaT in the presence of UDP-Gal and CMP-NeuAc. Subsequently, FucT and GDP-Fuc were added and incubated for another 48 h. After purification, the truncated PSGL-1 glycopeptides with or without sulfation, 36 and 37, were obtained with 43 and 25% yield, respectively. 3 Conclusion Recent advances in the area of enzymatic oligosaccharide synthesis are emerging from the identification and cloning of a large number of bacterial glycosyltransferases with many different donor, acceptor, and linkage specificities.
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