By Fitzroy Ambursley
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Parasitol Today 2:255-257 67. Gottesdiener K, Garcia-Anoveros J, Lee G-SM, Van der Ploeg LHT ( 1990) Chromosome organization of the protozoan Trypanosoma brucei. Mol Cell Bio110: 6079-6083 68. Dero B, Zampetti-Bosseler F, Pays E, Steinert M (1987) The genome and the antigen gene repertoire of Trypanosoma brucei gambiense are smaller than those ofT. b. brucei. Mol Biochem Parasitol26:247-256 69. Kanmogne GD, Bailey M, Gibson W (1997) Wide variation in DNA content among Trypanosoma brucei ssp. isolates.
The VSG gene expression sites The non-telomeric VSG genes have never been found to be transcribed. In contrast, most, if not all, telomeric VSG genes of the large chromosomes, excepting minichromosomes, appear to be potentially transcribable in situ. These telomeric loci are usually termed as the VSG gene expression sites. A current estimate for the number of expression sites in trypanosome bloodstream forms is about 20 . In addition, it is probable that a similar number of telomeric VSG genes, particularly from the largest chromosomes, is selectively transcribed in metacyclic forms [22, 23].
Workman PL (1969) The analysis of simple genetic polymorphisms. Human Biol41:97-114 77. Tibayrenc M, Kjellberg F, Ayala FJ ( 1990) A clonal theory of parasitic protozoa: The population structures of Entamoeba, Giardia, Leishmania, Naegleria, Plasmodium, Trichomonas and Trypanosoma and their medical and taxonomical consequences. Proc Natl Acad Sci USA 87: 24142418 78. Tibayrenc M, Kjellberg F, Arnaud J, Oury B, Breniere SF, Darda ML, Ayala FJ (1991) Are eucaryotic microorganisms clonal or sexual?