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Additional info for Handbook of Neurochemistry and Molecular Neurobiology 3rd Edition: Practical Neurochemistry Methods
The oxidized form is usually unstable and reacts further to form a stable compound that flows past the carbon electrode surface. Unfortunately, this is not always the case, with the stable oxidized form occasionally building up at the surfaces of the carbon electrode. This creates sensitivity problems and decreases the efficiency of the detector. However, the problem is usually overcome by regularly cleaning the carbon electrode surfaces, removing any oxidizable products. Eluents for EC detection must be electrochemically conductive, which is achieved by the addition of inert electrolytes (to maintain a baseline current) such as phosphate or acetate.
22 mm filters) and injecting 20 ml of the supernate directly onto the analytical column. The extraction recoveries averaged 79% for all the analytes of interest. The investigators reported that the method requires no sample pretreatment when using a Hisep analytical column and possesses the necessary sensitivity for pharmacokinetic and toxicological studies. A study by Larsimont and associates (1998) reported a method for the determination of promazine in human plasma. The RP‐HPLC assay with coulometric detection involved a single step LLE using a pentane/propanol mixture and separated on a Spherisorb CN analytical column.
The acidic aqueous layer may be basified again and the analyte reextracted into an organic layer. The organic layer may be taken to dryness for concentration of the analyte; the residue is then taken up in a solvent suitable for HPLC analysis. The overall recovery for this three‐step extraction process is often of the order of 60–80%. The sample volume required depends on the absolute concentration present and the selected method of analysis. 23 24 2 High‐performance liquid chromatographic analysis of psychotropic and endogenous compounds Disposable, prepackaged solid phase extraction (SPE) cartridges have introduced a wide range of phases that use polarity, hydrophobicity, or ionization as trapping mechanisms.